gene exp abcg2 hs01053790 m1  (Thermo Fisher)

Journal: Frontiers in Nutrition

Article Title: Oral L-carnitine supplementation lowers serum uric acid and is associated with renal and intestinal urate-handling responses in hyperuricemic mice

doi: 10.3389/fnut.2026.1866695

Figure Lengend Snippet: Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of ABCG2 in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: XOD (1:2000, Cat. No. 55156-1-AP, Proteintech), ADA (1:2000, Cat. No. 67870-1-Ig, Proteintech), OAT1 (1:2000, Cat. No. 26574-1-AP, Proteintech), OAT3 (1:2000, Cat. No. bs-0609R, Bioss), GLUT9 (1:1000, Cat. No. 26486-1-AP, Proteintech), ABCG2 (1:1000, Cat. No. 27286-1-AP, Proteintech), URAT1 (1:3000, Cat. No. 14937-1-AP, Proteintech), IκBα (1:1000, Cat. No. GB111509 , Servicebio), NF-κB p65 (1:2000, Cat. No. 66535-1-Ig, Proteintech), phospho-NF-κB p65 (1:2000, Cat. No. ZHS1565, ZhongHe) and β-actin (1:8000, Cat. No. 66009-1-Ig, Proteintech).

Techniques: Morphology, Expressing, Staining, Tissue, Immunofluorescence, Analysis

Journal: STAR Protocols

Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

doi: 10.1016/j.xpro.2026.104361

Figure Lengend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.

Techniques: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence